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KMID : 0385219970070010017
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Korean Journal of Gerontology
1997 Volume.7 No. 1 p.17 ~ p.24
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Ameliorating Effects of Calcium·Magnesium Lithospermate B on Cognitive Deficiencies in Senescence-Accelerated Mouse
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Takashi Arima
Matsuno Junko
Hori Hitomi
Kitamura Yoshihisa
Tsuneo Namba
Masao Hattori
Shigeroshi Kadota
Yasuyuki Nomura
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Abstract
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Effect of long-term treatment with calcium/magnesium lithospermate B(LSB), a major ingredient of Dan-Shen (salviae miltiorrhizase radix) methanol extract (DME), on memory and learning in the scenescence-accelerated mouse was investigated by means of Morris’s water maze task. LSB treatment significantly decreased both escape latency and path length in the P8 strain of senescence-accelerated mouse (SAMP8), which strain spontaneously develops learning deficits. These results suggest that LSB treatment improved spatial learning. In neurochemical investigation, no effect on choline acetyltransferase activity or [3H]QNB (quinuclidinyl benzilate) binding was observed in the cortex and hippocampus. Similarly, no effect was observed in [3H]MK-801 ((+)-5-methyl-10,11-dihydro-5H-dibenzo [a,b]-cyclohepten-5,10-imine maleate) binding, [3H]NNA (NG-nitro-L-arginine) binding, or [3H]PDBu (phorbol 12,13-dibutyrate) binding in the membrane fractions. However [3H]PDBu binding in the cytosolic fraction was increased in the hippocampus. Thus our data suggest that long-term LSB treatment improves spatial learning in SAMP8. As to the mechanism, LSB treatment alters the hippocampal functions mediated by protein kinase C (PKC).
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KEYWORD
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Lithospermate B, SAMP8, spatial learning, NMDA(N-methyl-D-aspartate), PKC(protein kinase C), nitric oxide synthase
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